A depiction of the Fountain of Youth by Lucas Cranach the Elder (1472 – 1553)

EGA®-Nicotinamide Mononucleotide (NMN) can be dissolved and drunk in water, a Fountain of Youth.

The dream of a “Fountain of Youth” dates back to writings of Herodotus (Book III:23) who lived between 484–425 BC. The tale of such a fountain has been recounted across the world now for thousands of years. The Fountain of Youth is a legendary spring that supposedly restores the youth of anyone who drinks its waters. Juan Ponce de Leon, the explorer and first Governor of Puerto Rico, dreamed of this Fountain of Youth and searched for it on a 1513 exploration of what is now Florida.

In 450 BC Herodotus spoke of the longevity, physical prowess, beauty, and general youthfulness of a group he called the Macrobians. The Macrobians were a people that thrived during the first millennium B.C. Herodotus attributes no magical qualities to a fountain of youth and paints no portrait like the one of modern fantasy. He suggests, rather, that given their strength and longevity, the Macrobians likely reaped some unique nutrition from their waters. The Macrobians most likely lived in the vicinity of the Rift Valley of what is now Ethiopia.

In 1901

Elie Metchnikoff wrote the Metchnikoff’s Hypothesis of Aging

In 1908

Max Rubner wrote the Rate of Living Theory of Aging

In 1928

Raymond Pearl updated the Rate of Living Theory of Aging

In 1935

Clive Mc Cay first observed calorie restriction elongating life.
(Calorie Restriction Story of Aging)

In 1956

Denham Harman proposed the “free-radical theory of aging”. A free-radical is any atom or molecule that has a single unpaired electron in an outer shell. Free radical damage is associated with damage to the biological molecules in cells. NMN is a precursor of NAD+ that is found in every cell. NAD+ is involved in reduction / oxidation (redox) reactions carrying electrons from one reaction to another. NAD+ accepts electrons from molecules and NADH donates electrons to molecules.
(Free-Radical Story of Aging)

In 1959

Leo Szilard wrote The Somatic Mutation Theory of Aging

In 1967

Boris Vanyushin showed that DNA (of salmon) loses methylation with age (of rats 1973, of cows 1978). He also showed DNA methylation was tissue (cell) and species specific.
(Methylation Story of Aging)

In 1972

Deham Harman proposed that free-radicals also called reactive oxygen species (ROS) from mitochondria were the primary cause of aging.
(Free-Radical Story of Aging)

In 1986

Richard Weindruch confirmed aging could be slowed by using calorie restriction in mice. Eating 2/3 the calories of a normal diet allowed mice to live 40% longer.
(Calorie Restriction Story of Aging)

In 1991

Richard Weindruch confirmed aging could be slowed by using calorie restriction in mice. Eating 2/3 the calories of a normal diet allowed mice to live 40% longer.

In 1998

Boris Vanyushin showed that an antioxidant (BHT) induced de novo DNA methylating enzymes, stimulated transcription of p53, and modulated DNA methylation in rats.
(Methylation and Free-Radical Story of Aging)

In 2000

Leonard Guarente realized sirtuins were nutrient sensors and they might mediate the effects of calorie restriction.
(Calorie Restriction Story of Aging)

In 2000

Shin-Ichiro Imai showed sirtuins were NAD-dependent deacetylases.
(Calorie Restriction Story of Aging)

In 2002

Kevin Bitterman showed nicotinamide inhibited Sirtuins in a noncompetitive manner with NAD+.
(Calorie Restriction Story of Aging)

In 2007

Claudio Franceschi wrote the Inflammaging Theory of Aging.

In 2008

Katheryn Ramsey and Katheryn Mills showed nicotinamide mononucleotide (NMN) produced age reversal effects in mice.
(Calorie Restriction Story of Aging)

In 2009

Brock Christensen showed DNA methylation was decreased outside CpG islands and DNA methylation increased inside CpG islands with age in humans.
(Methylation Story of Aging)

In 2010

Gaelle Laurent wrote the Retrotransposon Theory of Aging

In 2011

Jun Yoshino and Katheryn Mills showed the benefits of nicotinamide mononucleotide (NMN) was getting past the rate limiting enzyme in mammalian NAD+ biosynthesis called nicotinamide phosphoribosyltransferase (NAMPT) and that NMN is converted into NAD+ efficiently in mice. NMN is converted into NAD+ in each cell’s nucleus, cytoplasm, and mitochondria by a set of 3 enzymes called mononucleotide adenylyl transferases (MNMAT 1,2,3), each specializing in these locations.
(Calorie Restriction Story of Aging)

In 2012

Leonidas Chouliaras showed calorie restriction prevents the age-related changes of methylation of DNA in mice.
(Calorie Restriction and Methylation Story of Aging)

In 2012

Hassina Massudi found a link between oxidative stress from ROS, aging and a decline in NAD+ levels in human tissue.
(Free-Radical Story of Aging)

In 2012

Rajindar Sohal wrote the Redox Stress Hypothesis of Aging.
(Free-Radical Story of Aging)

2013

Kathrin Schmeisser and Johannes Mansfeld showed sirtuin lifespan extension depends on methylation of nicotinamide by amine N-methyltransferase (ANMT) making 1-methylnicotinamide to prevent nicotinamide’s inhibition of situins. Nicotinamide decreases life span at high dosage.
(Calorie Restriction and Methylation Story of Aging)

2013

The reversal of age in mice was scientifically confirmed in a publication of the scientific journal Cell by lead author Ana Gomes working in David Sinclair’s laboratory at Harvard Medical School. This research used nicotinamide mononucleotide (NMN) to reverse aging in mice.
(Methylation Story of Aging)

In 2014

Egaceutical Corporation was founded to bring nicotinamide mononucleotide (NMN) to the market place for human use.

In 2014

Egaceutical Corporation started a 12 person study using EGA® for human age reversal. EGA® is a water based product that includes three ingredients: nicotinamide mononucleotide (NMN) a compound that turns into cellular NAD+, a compound that increases methyl donor, SAM, for cellular methylation, and a compound that turns on antioxidant defense activation, Nrf2, to increase cellular antioxidant enzymes that decrease oxidation and increase cell reduction.

In 2015

EGA® study results showed age reversal in 12 human males.
(Unified Theory of Aging)

In 2015

Egaceutical Corporation filed the first patent application for human age reversal. The patent application was to protect its product EGA®.
(Unified Theory of Aging)

In 2017

The PCT published the first patent application for age reversal in humans, PCT/US2016/055173, called “RESETTING BIOLOGICAL PATHWAYS FOR DEFENDING AGAINST AND REPAIRING DETERIORATION FROM HUMAN AGING”. The inventor is Joel T. Huizenga of Egaceutical Corporation.
(Unified Theory of Aging)

Timeline Of Advancements In Age Reversal

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